Capillary zone electrophoresis

Journal Article · Fri Oct 21 04:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6623076· OSTI ID:5384292

Jorgenson, J W; Lukacs, K D

Zone electrophoresis in capillaries is a technique complementary to electrophoresis in supporting media, and each approach has its own particular advantages. Efficient heat transfer from small-diameter capillaries permits use of unusually high voltages, resulting in both high resolution and rapid analysis. Capillaries also seem well suited for automation. Our present electromigration injection technique is relatively straightforward and should be simple to automate. Capillaries are reusable, which is an advantage over gels. On-line electronic detection permits good quantification, further enhancing possibilities for fully automatic operation. The greatest obstacle to further development and utilization of capillaries is the requirement of extremely sensitive detectors, and more types of detectors with higher sensitivity are greatly needed. A better understanding of capillary surface modification will also be important, both for improved capillary surface deactivation and for better control over electroosmotic flow. Capillaries should provide an ideal system in which to explore nonaqueous separation media. The prospects for nonaqueous media in electrophoresis are similar to those in electrochemistry, and capillaries should prove an excellent system in which to begin their study. 18 refs., 8 figs.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 5384292
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 222:4621; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
DOCUMENT TYPES
ELECTROPHORESIS
ORGANS
REVIEWS