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Decrease in platelet activating factor stimulated phosphoinositide turnover during storage of human platelets in plasma

Conference · · Federation Proceedings, Federation of American Societies for Experimental Biology; (USA)
OSTI ID:5052674
;  [1]
  1. Univ. of Missouri School of Medicine, Columbia (USA)
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Human platelet concentrate from the American Red Cross Blood Center was stored at 24{degree}C in a shaker and aliquots were taken out at time intervals aseptically. Platelet activating factor (PAF) stimulated turnover of phosphoinositide (PPI) was monitored by assaying {sup 32}P incorporation into phosphoinositides using platelet rich plasma (PRP). Platelets in PRP were incubated with 1 {times} 10{sup {minus}7} M PAF at 37{degree}C with gentle shaking and after 5 min their lipids were extracted and analysed by TLC for {sup 32}P-phosphoinositides. The percent stimulation of {sup 32}P incorporation by PAF (over control) into PPI was approximately 250, 100, 60, 25 and 20 on days 1, 2, 3, 5 and 6, respectively. This indicated a dramatic decrease in PAF responsive turnover of platelet PPI during storage. These findings have important implications in relation to PAF receptor activity and viability of platelets at different periods of storage.

OSTI ID:
5052674
Report Number(s):
CONF-870644--
Journal Information:
Federation Proceedings, Federation of American Societies for Experimental Biology; (USA), Journal Name: Federation Proceedings, Federation of American Societies for Experimental Biology; (USA) Vol. 46:6; ISSN 0014-9446; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLASMA
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL ACTIVATION
DAYS LIVING RADIOISOTOPES
DRUGS
ESTERS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
REACTION KINETICS
RESPONSE MODIFYING FACTORS
STORAGE
TRACER TECHNIQUES
VIABILITY