Response to platelet-activating factor in human platelets stored and aged in plasma. Decrease in aggregation, phosphoinositide turnover, and receptor affinity

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1989.29689318453.x· OSTI ID:5603864

Shukla, S D; Morrison, W J; Klachko, D M

Human platelet concentrates were stored in polyolefin bags at 22 to 24 degrees C on a horizontal shaker for up to 8 days. At different intervals, aliquots of platelet-rich plasma (PRP) were removed aseptically and five variables, i.e., platelet counts, morphology, platelet-activating factor (PAF)-stimulated aggregation, phosphoinositide turnover, and (3H)PAF binding to platelet receptors, were studied. The number of platelets did not change during the 8 days of storage. Scanning electron microscopy of the platelets revealed a gradual morphologic change from biconcave flat discs to irregular, crenated forms. The PAF-induced aggregation of platelets declined with time of storage. A decrease to 50 percent of the Day 1 aggregatory response to PAF was evident on Day 2, and there was a further decline to about 20 percent by Day 6. Similarly, PAF receptor-coupled phosphoinositide turnover, as monitored by 32P incorporation into individual phosphoinositides, decreased dramatically with storage. After 2 to 3 days of storage, the phosphoinositide turnover was reduced to 50 percent of the original response, and it continued to decline to about 25 percent of original response by Day 5 or 6. The binding of (3H)PAF to washed human platelets indicated subtle changes between Days 2 and 4, which became more noticeable by Day 6. These results have raised the possibility of changes in the number of the receptors and/or their affinity for the ligand during storage. We conclude that although the number of platelets was maintained during storage for 8 days, a general deterioration of their responses to PAF occurred at the levels of cell surface receptor, transmembrane signaling (phosphoinositide turnover), and response (aggregation).

• Research Organization: Univ. of Missouri-Columbia (USA)
• OSTI ID: 5603864
• Journal Information: Transfusion (Philadelphia); (United States), Journal Name: Transfusion (Philadelphia); (United States) Vol. 29:6; ISSN TRANA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD COAGULATION FACTORS
BLOOD COUNT
BLOOD PLATELETS
BODY FLUIDS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON MICROSCOPY
ESTERS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
METABOLISM
MICROSCOPY
MORPHOLOGICAL CHANGES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SCANNING ELECTRON MICROSCOPY
STORAGE LIFE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES