Unscheduled synthesis of DNA and poly(ADP-ribose) in human fibroblasts following DNA damage
Journal Article
·
· J. Supramol. Struct. Cell. Biochem.; (United States)
Unscheduled DNA synthesis has been measured in human fibroblasts under conditions of reduced rates of conversion of NAD to poly)ADP-ribose). Cells heterozygous for the xeroderma pigmentosum genotype showed normal rates of uv induced unscheduled DNA synthesis under conditions in which the rate of poly(ADP-ribose) synthesis was one-half the rate of normal cells. The addition of theophylline, a potent inhibitor of poly(ADP-ribose) polymerase, to the culture medium of normal cells blocked over 90% of the conversion of NAD to poly(ADP-ribose) following treatment with uv or N-methyl-N'-nitro-N-nitro-soguanidine but did not affect the rate of unscheduled DNA synthesis.
- Research Organization:
- North Texas State Univ./Texas Coll. of Osteopathic Medicine, Denton
- OSTI ID:
- 5046868
- Journal Information:
- J. Supramol. Struct. Cell. Biochem.; (United States), Journal Name: J. Supramol. Struct. Cell. Biochem.; (United States) Vol. 17:1; ISSN JSSBD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Poly(ADP-ribose) synthesis following DNA damage in cells heterozygous or homozygous for the xeroderma pigmentosum genotype
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Symposium cellular response to DNA damage the role of poly(ADP-ribose) poly(ADP-ribose) in the cellular response to DNA damage
Journal Article
·
Sat Jan 24 23:00:00 EST 1981
· J. Biol. Chem.; (United States)
·
OSTI ID:6056192
Poly(ADP-ribose) biosynthesis and suicidal NAD+ depletion following carcinogen exposure of mammalian cells
Journal Article
·
Thu Aug 14 00:00:00 EDT 1986
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:5002806
Symposium cellular response to DNA damage the role of poly(ADP-ribose) poly(ADP-ribose) in the cellular response to DNA damage
Conference
·
Mon Dec 31 23:00:00 EST 1984
· Radiat. Res.; (United States)
·
OSTI ID:5930571
Related Subjects
550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CARDIOVASCULAR AGENTS
CELL CULTURES
COENZYMES
CONNECTIVE TISSUE CELLS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
MAMMALS
MAN
NAD
NITROSO COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
SOMATIC CELLS
SYNTHESIS
THEOPHYLLINE
ULTRAVIOLET RADIATION
VASODILATORS
VERTEBRATES
XANTHINES
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CARDIOVASCULAR AGENTS
CELL CULTURES
COENZYMES
CONNECTIVE TISSUE CELLS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
MAMMALS
MAN
NAD
NITROSO COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
SOMATIC CELLS
SYNTHESIS
THEOPHYLLINE
ULTRAVIOLET RADIATION
VASODILATORS
VERTEBRATES
XANTHINES