Heart cells in culture: a model of myocardial iron overload and chelation
The effect of iron loading and chelation was studied in heart cell cultures obtained from newborn rats. Radioactive iron uptake per 2 X 10(6) cells/24 hr was 3.8% for /sup 59/Fe-transferrin, 15.8% for /sup 59/Fe-ferric ammonium citrate (FeAC) at 20 micrograms Fe/ml in 20% serum, and 37.1% for /sup 59/FeAC at 20 micrograms Fe/ml in serum-free medium. About one third of the cellular radioactive iron was in ferritin and the rest in an insoluble lysosomal fraction. Iron uptake was almost completely inhibited by reducing the incubation temperature from 37 degrees C to 10 degrees C. Intracellular concentrations of malonyldialdehyde (MDA) were doubled after 15 minutes of iron loading and reached maximal concentrations at 3 hours. Conversely, iron mobilization by deferoxamine at concentrations ranging from 0.025 mmol/L to 0.3 mmol/L resulted in normalization of cellular MDA concentrations, in direct proportion to the amounts of iron removed. These findings indicate that cultured myocardial cells are able to assimilate large amounts of nontransferrin iron and that iron uptake and mobilization are associated with striking changes in lipid peroxidation as manifested by the respective increase and decrease in cellular MDA concentrations.
- Research Organization:
- Hebrew Univ. Hadassah Medical School, Jerusalem, Israel
- OSTI ID:
- 5018847
- Journal Information:
- J. Lab. Clin. Med.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
IRON
BIOLOGICAL EFFECTS
UPTAKE
LIPIDS
METABOLISM
ALDEHYDES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
CELL CULTURES
CHELATING AGENTS
DEFEROXAMINE
FERRITIN
IRON 59
LABELLED COMPOUNDS
MYOCARDIUM
RATS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
TRANSFERRIN
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
COMPLEXES
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
HEART
INTERMEDIATE MASS NUCLEI
IRON COMPLEXES
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METALLOPROTEINS
METALS
MUSCLES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550501 - Metabolism- Tracer Techniques