Stimulation of prostacyclin synthesis in rats by phenobarbital treatment
Prostacyclin (PGI/sub 2/), synthesized in the endothelial cells of arteries, is known to inhibit the aggregation of platelets and hence thrombosis in blood. Low levels of PGI/sub 2/ have been observed in coronary heart disease which is associated with low levels of HDL in blood. Recently, it has been shown that synthesis of PGI/sub 2/ in vitro, is stimulated by HDL. Hence it seems likely that higher level of HDL in blood would increase the level of PGI/sub 2/ in blood. Since phenobarbital treatment is known to increase blood HDL levels in humans and animals, this study was undertaken to determine the effect of phenobarbital treatment on the synthesis of PGI/sub 2/. Coronary vascular microsomes were prepared from Sprague Dawley rats treated with phenobarbital for two days. The PGI/sub 2/ synthesizing activity was assayed by incubating these microsomes with 1-/sup 14/C-arachidonic acid and by determining the /sup 14/C-activity recovered in 6-ketoprostaglandin F/sub 1..cap alpha../, the stable decomposition product of PGI/sub 2/. Phenobarbital treatment increased the synthesis of PGI/sub 2/ nearly 2-fold. Addition of phenobarbital did not increase PGI/sub 2/ synthesis in control microsomes; however, the synthesis was increased by HDL. Thus, it appears that the observed increase in PGI/sub 2/ synthesis resulting from phenobarbital treatment was partly, if not totally, due to the increase in blood HDL level.
- Research Organization:
- Kent State Univ., OH
- OSTI ID:
- 5018034
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANESTHETICS
ANIMAL TISSUES
ANIMALS
ANTICONVULSANTS
ARACHIDONIC ACID
AZINES
BARBITURATES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISEASES
DRUGS
ENDOTHELIUM
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
MAMMALS
MATERIALS
MICROSOMES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
PATHOGENESIS
PHENOBARBITAL
PROSTAGLANDINS
PROTEINS
PYRIMIDINES
RATS
RODENTS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES