ATPase and morphologic changes induced by UVB on Langerhans cells in guinea pigs

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12276550· OSTI ID:5017438

Hanau, D; Fabre, M; Lepoittevin, J P; Stampf, J L; Grosshans, E; Benezra, C

The authors have devised, in guinea pigs, an improved ATPase technique which enables one to proceed from light to electron microscope study while preserving, on the ultrastructural level, the various membranous structures, in particular the Langerhans cell (LC) granules. Using this method, they have been able to confirm the action of acute, low-dose UVB on the surface enzymatic marker, ATPase. Moreover, this study has shown that the ATPase-negative LC contain abnormal LC granules or, more often, are deficient in LC granules. In a previous work, the authors have shown that, after epicutaneous application of a hapten, one successively observes an extensive adsorptive pinocytosis process, the disappearance of the membranous ATPase system, and the appearance of LC granules in the cytoplasm. Therefore, the authors may suppose that, after UVB irradiation, the disappearance of the ATPase system and/or the possible alteration of the adsorptive pinocytosis process interrupts or alters the formation of LC granules. These successive events might play a vital role in the formation of the hapten--carrier protein-Ia antigen complex. In their absence in a large number of LC, following UV irradiation, epicutaneous application of a hapten would lead to the development of a state of immune tolerance.

• Research Organization: Universite Louis Pasteur, Strasbourg, France
• OSTI ID: 5017438
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 2; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ANIMAL CELLS
ANIMALS
ATP-ASE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
ENDOCRINE GLANDS
ENZYMES
GLANDS
GUINEA PIGS
HYDROLASES
IMMUNE REACTIONS
MAMMALS
MEMBRANES
MICROSCOPY
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PHOSPHOHYDROLASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
ULTRASTRUCTURAL CHANGES
ULTRAVIOLET RADIATION
VERTEBRATES