Oxygen intermediates are involved in ultraviolet radiation-induced damage of Langerhans cells
Experiments were conducted to determine whether ultraviolet (UV) radiation exerts its effect through the generation of oxygen intermediates on Langerhans cells (LC). Guinea pigs were exposed to one single dose of UVB (0.9-2.7J/cm2), and biopsy specimens were taken 5 days after the irradiation. The population of LC was evaluated using ATPase-stained epidermal sheets. These exposures reduced the number of LC to 20-25% of the original density. On the other hand, superoxide dismutase (SOD) (0.02-0.2 mg), a scavenger of superoxide anion, which had been injected intradermally just before UV radiation, significantly prevented the depletion of LC, although not completely (37-40% of the original density). The injection immediately after the exposure was still significantly effective, but less so. Other scavengers of oxygen intermediates including catalase, D-mannitol, and L-histidine revealed no detectable effect. A single exposure of UVB at doses of 0.3-0.6 J/cm2 did not deplete the ATPase-positive LC. However, the same dose of UVB reduced the number of LC to 70%, when exposed after the injection of an SOD inactivator, diethyldithiocarbamate, possibly due to inactivation of physiologically existing SOD. These observations indicate that oxygen intermediates such as superoxide anion or its subsequent species are generated by UV radiation exposure and damage the epidermal LC.
- Research Organization:
- Kyoto Univ., Japan
- OSTI ID:
- 6328276
- Journal Information:
- J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 6; ISSN JIDEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition of epidermal Langerhans cell function by low dose ultraviolet B radiation. Ultraviolet B radiation selectively modulates ICAM-1 (CD54) expression by murine Langerhans cells
In vitro effect of UV radiation on immune function and membrane markers of human Langerhans cells
Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
CATALASE
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ELEMENTS
ENDOCRINE GLANDS
ENZYMES
GLANDS
GUINEA PIGS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HORMONES
IMIDAZOLES
INSULIN
IRRADIATION
MAMMALS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN
PANCREAS
PEPTIDE HORMONES
PEROXIDASES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SUPEROXIDE DISMUTASE
ULTRAVIOLET RADIATION
VERTEBRATES