Distribution of the carcinogenic tryptophan pyrolysis product Trp-P-1 in control, 9-hydroxyellipticine and. beta. -naphthoflavone pretreated mice

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/4.10.1291· OSTI ID:5014682

Brandt, I ^[1]; Gustafsson, J A; Rafter, J ^[2]

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1. Uppsala Biomedical Centre (Sweden). Dept. of Pharmacology
2. Kungliga Karolinska Mediko-Kirurgiska Inst., Stockholm (Sweden)

Autoradiograms obtained 1-4 h after i.v. injection of the /sup 14/C-labelled carcinogenic tryptophan pyrolysis product Trp-P-1 to albino and pigmented mice showed a pronounced uptake of radioactivity in the lymphatic system, in the endocrine system and in the liver, kidney medulla and brain. High radioactivity was present in the excretory pathways, predominantly in the bile/intestinal contents. At longer post-injection times most of the labelled substance had left the tissues, except for the liver. Trp-P-1 is known to be activated by cytochrome P-448. The uptake of radioactivity in the liver could be reduced by pretreatment with the cytochrome P-448 inhibitor 9-hydroxyellipticine suggesting that the observed accumulation of radioactivity in the liver was partly due to metabolites of Trp-P-1. After pretreatment with the cytochrome P-448 inducer ..beta..-naphthoflavone, the administration of Trp-P-1 resulted in a highly selective accumulation of radioactivity in the lung parenchyma, exceeding all other tissues. ..beta..-Naphthoflavone pretreatment also increased the uptake of radioactivity in the kidney cortex and small intestinal mucosa. As indicated by a high labelling of the pigmented tissues of the maternal and fetal eye, the carcinogen and/or its metabolites were accumulated in melanin.

• OSTI ID: 5014682
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:10; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AROMATICS
AUTORADIOGRAPHY
AZAARENES
AZOLES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARCINOGENS
DISTRIBUTION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VIVO
INDOLES
LABELLED COMPOUNDS
MAMMALS
MICE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYROLYSIS PRODUCTS
PYRROLES
RESPONSE MODIFYING FACTORS
RODENTS
TISSUE DISTRIBUTION
TRYPTOPHAN
VERTEBRATES