Cadmium-induced lung injury: cell kinetics and long-term effects

Journal Article · Sun Sep 15 04:00:00 EDT 1985 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(85)90078-X· OSTI ID:5014259

Martin, F M; Witschi, H P

The effects of exposure to CdCl/sub 2/ aerosols followed by hyperoxia were studied in mouse lung. Special emphasis was placed on analysis of cell proliferation following injury. Male Balb/c mice were exposed to aerosols of 4.9 micrograms Cd/liter for 1 hr while controls were exposed to water aerosols. Immediately after, half of each group was placed in 80% O/sub 2/ for 6 days, while the rest were left in room air. Three endpoints were used to assess lung injury; measurement of hydroxyproline, (/sup 14/C)thymidine incorporation into DNA, and histopathology. Parenchymal and bronchiolar labeling indices were determined following autoradiography. A 1-hr exposure to CdCl/sub 2/ aerosols caused marked cell proliferation in the lung with the peak of cell labeling occurring at Day 5. In animals exposed to both CdCl/sub 2/ + 80% O/sub 2/, the cell labeling peak was delayed until Day 9. Cell differentiation studies showed a delay in the peak of type II epithelial cell and endothelial cell division when CdCl/sub 2/ exposure was followed by the 80% O/sub 2/ treatment. On Day 15 most of the labeled cells were identified as interstitial cells in both treated groups. Bronchiolar cell labeling was suppressed at the early time period in the Cd + O/sub 2/ group. With time, the histologically visible lung lesions tended to resolve in animals exposed to CdCl/sub 2/ or CdCl/sub 2/ and 80% O/sub 2/, whereas total pulmonary hydroxyproline remained at all times (3, 6, and 12 months) significantly higher in Cd-treated animals when compared to controls. It was concluded that acute lung injury by a toxic inhalant can be amplified if there is an initial delay in pulmonary cell proliferation following an acute insult.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 5014259
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 2; ISSN TXAPA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
AZOLES
BODY
BRONCHI
CADMIUM
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL DIVISION
CELL PROLIFERATION
COLLOIDS
DISPERSIONS
DNA
ELEMENTS
ENDOTHELIUM
EPITHELIUM
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYPROLINE
INHALATION
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
METALS
MICE
NONMETALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN
PATHOLOGICAL CHANGES
PYRIMIDINES
PYRROLES
PYRROLIDINES
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SOLS
THYMIDINE
TISSUES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES