Apolipoprotein B is a calcium binding protein
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Human hepatocarcinoma Hep G2 cells were grown in culture medium containing (/sup 45/Ca/sup 2 +/). The secreted lipoproteins of d < 1.063 g/ml and d 1.063-1.21 g/ml were isolated from the culture media and analyzed by 3.3% and 7% SDS-polyacrylamide gel electrophoresis. Radioactivity profiles of (/sup 45/Ca) from the gels showed that the peak of radioactivity corresponded to the apolipoprotein B band. The molar ratio of the incorporated (/sup 45/Ca/sup 2 +/) and apolipoprotein B was close to unity. No radioactivity was found associated with any other secreted apolipoproteins. To confirm these findings, apolipoprotein B-containing lipoproteins were precipitated with anti-apolipoprotein B and high density lipoproteins were precipitated with anti-apolipoprotein A-I. Only the former precipitate was radioactive. These results suggest that apolipoprotein B is a calcium binding protein.
- Research Organization:
- Univ. of Oklahoma Health Sciences Center, Oklahoma City
- OSTI ID:
- 5009212
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM 45
CALCIUM ISOTOPES
CATIONS
CHARGED PARTICLES
DATA
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROPHORESIS
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
HEPATOMAS
INFORMATION
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
LIVER CELLS
NEOPLASMS
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
PRECIPITATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM 45
CALCIUM ISOTOPES
CATIONS
CHARGED PARTICLES
DATA
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROPHORESIS
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
HEPATOMAS
INFORMATION
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
LIVER CELLS
NEOPLASMS
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
PRECIPITATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES