Changes in plasma apolipoproteins following whole-body irradiation in rabbit

Journal Article · Tue Sep 01 04:00:00 EDT 1981 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575524· OSTI ID:6142226

Feliste, R ^[1]; Dousset, N; Carton, M; Douste-Blazy, L

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1. INSERM, Toulouse, France

Male New Zealand white rabbits were whole-body-irradiated with a linear electron accelerator at 800 rad (LD/sub 50/ in 30 days). This treatment induced a pronounced hypertriglyceridemia. The apoprotein composition of very low density lipoproteins (VLDL, d < 1.006 g/ml) and high-density lipoproteins (HDL, d = 1.063 - 1.21 g/ml) from irradiated rabbits was studied and compared to those of normal rabbits. Significant changes were observed in both very low density apolipoproteins and high-density apolipoproteins. (1) In the VLDL fraction from irradiated rabbits, there appeared in high proportion two apolipoproteins with electrophoretic mobility in urea/polyacrylamide gels similar to apoA-I and A-II but which were distinctly different in their apparent molecular weights, their isoelectric points, and their amino acid composition from these latter proteins. These proteins had apparent molecular weights of about 10,000. They focused into three bands with pI values of 6.1, 6.4, and 6.6. Their amino acid composition was characterized by a very low content of threonine and serine and a high content of aspartic acid, glycine, alanine, and arginine. In addition, a marked increase of an apolipoprotein with an apparent molecular weight of about 43,000 and with an amino acid composition similar to rat apoA-IV was also observed in rabbit VLDL after irradiation. Apolipoprotein C constituents with slowmobility decreased significantly. (2) The irradiated rabbit HDL apolipoproteins showed an important increase of the proteins with molecular weight 10,000 and isoelectric points 6.1, 6.4, and 6.6. Compared to normal rabbit HDL apolipoproteins, a significant decrease of apoA-IV occurred. These modifications were also observed with lower radiation doses (200 and 400 rad).

• OSTI ID: 6142226
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 87:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
APOLIPOPROTEINS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CHEMICAL COMPOSITION
COMPARATIVE EVALUATIONS
DENSITY
DOSES
ESTERS
EXTERNAL IRRADIATION
IRRADIATION
LIPIDS
LIPOPROTEINS
MAMMALS
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
PHYSICAL PROPERTIES
PLASMA
PROTEINS
RABBITS
RADIATION DOSES
RADIATION EFFECTS
SYNTHESIS
TRIGLYCERIDES
VERTEBRATES
WHOLE-BODY IRRADIATION