Serum and urinary lipoproteins in the human nephrotic syndrome: evidence for renal catabolism of lipoproteins

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Metabolism; (United States)

DOI:https://doi.org/10.1016/0026-0495(82)90062-2· OSTI ID:6816302

Shore, V G; Forte, T; Licht, H; Lewis, S B

The urinary excretion of lipoproteins and the possibility of catabolic alterations on glomerular filtration were investigated in four nephrotic subjects difering in etiology, serum lipoprotein profile, and 24 hr urinary output of protein and lipids. The apolipoproteins and lipoproteins of urine were compared with those of serum with respect to distribution profile, physical properties, and composition. As expected from molecular sieving effects during glomerular filtration, the urinary HDL were more abundant than the lower density lipoproteins even when the plasma LDL was elevated markedly. Intact apolipoproteins were not found in the concentrated urinary fraction isolated by ultrafiltration between the limits of 10/sup 4/ and 5 x 10/sup 4/ daltons. On the basis of immunoreactivity, gel electrophoresis, and amino acid composition, apolipoproteins B and AI are the major and minor proteins, respectively, of urinary LDL, and apo B is the major protein of the urinary IDL and VLDL. Apolipoproteins AI, AII, CI, CIII, and possibly AIV were isolated from the urinary HDL. As much as 20% of the protein moiety of the urinary HDL appeared to be large apolipoprotien fragments with molecular weights and isoelectric points similar to those of apo CII and apo CIII. The lower density classes of urinary lipoproteins also appeared to have lost apo E and apo C's and to have undergone partial proteolysis.

• Research Organization: Lawrence Livermore National Lab., CA
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6816302
• Journal Information: Metabolism; (United States), Journal Name: Metabolism; (United States) Vol. 31:3; ISSN MTBMA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY
BODY FLUIDS
CATABOLISM
CHEMISTRY
CLEARANCE
DISEASES
EXCRETION
FILTRATION
GLOMERULI
KIDNEYS
LIPIDS
LIPOPROTEINS
MATERIALS
METABOLISM
NEPHRITIS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGY
PATIENTS
PROTEINS
SEPARATION PROCESSES
ULTRAFILTRATION
URINE
UROGENITAL SYSTEM DISEASES
WASTES