Apolipoprotein B synthesis in humans: liver synthesizes only apolipoprotein B-100
Journal Article
·
· Metab., Clin. Exp.; (United States)
Apolipoprotein (apo) B-100 and B-48 are prominent apolipoproteins in VLDL, IDL, and chylomicrons. Organ cultures of normal adult human liver were established to ascertain the form of apo B synthesized by hepatocytes in humans. Human liver was minced and incubated in 15 mL methionine-free RPMI-1640 medium with 10% dialyzed fetal calf serum plus 250 microCi /sup 35/S-methionine for eight hours at 37 degrees C. Lipoproteins secreted by the liver were isolated by ultracentrifugation and the content of newly synthesized apo B determined by quantitation of radioactivity in the apoB-100 and apoB-48 bands after separation by 3% NaDodSO/sub 4/ gel electrophoresis. In the eight-hour period, 2.5% to 3.2% of added /sup 35/S-methionine was secreted in TCA-precipitable protein of which 0.34% was apo B. Ninety-nine percent of the apo B in VLDL, IDL, and LDL was in the apo B-100 electrophoretic band. No significant radioactivity was detected in the apo B-48 electrophoretic band. Eighty-nine percent of the total radioactivity of apo B-100 was in VLDL with 3% and 8% in IDL and LDL, respectively. These results establish that adult human liver in organ culture synthesizes apo B-100 but not apo B-48.
- Research Organization:
- National Cancer Institute, Bethesda, MD
- OSTI ID:
- 5098450
- Journal Information:
- Metab., Clin. Exp.; (United States), Journal Name: Metab., Clin. Exp.; (United States) Vol. 8; ISSN METAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CENTRIFUGATION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
LIVER
MAMMALS
MAN
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUE CULTURES
TRACER TECHNIQUES
ULTRACENTRIFUGATION
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CENTRIFUGATION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
LIVER
MAMMALS
MAN
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUE CULTURES
TRACER TECHNIQUES
ULTRACENTRIFUGATION
VERTEBRATES