Pulmonary metastasis after bleomycin-induced endothelial injury and repair

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5006848

Adamson, I Y.R.; Young, L; Orr, F W

Injury to the endothelial barrier is thought to enhance the localization and metastasis of circulating tumor cells. This hypothesis was tested by inducing pulmonary endothelial injury in C57bl/6 mice by injecting a single IV dose of bleomycine (120 mg/kg). After 5 days, severe endothelial injury was demonstrated by morphology and by increased levels of protein in lung lavage fluid. When /sup 131/I-iododeoxyuridine labeled syngeneic fibrosarcoma cells were injected IV at this time, a 9 fold increase in their localization was detected 24 hrs later in bleomycin-treated lungs compared to saline controls. By EM, tumor cells were observed at sites of denuded vascular basement membrane. There was also a significant increase in subsequent gross metastases and percentage of lung occupied by tumor in the bleomycin group. Animals examined 10 days after bleomycin showed less endothelial damage and a smaller increase in tumor cell localization and metastases. At 21 days, when endothelial structure and alveolar protein levels had returned to normal, and at 6 weeks, when there was focal fibrosis, no increase in tumor cell localization or metastases was found. It is concluded that damage to the pulmonary endothelium is a key factor in enhancing the trapping of circulating tumor cells and increasing metastatic tumor growth.

• Research Organization: Univ. of Manitoba, Winnipeg
• OSTI ID: 5006848
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BLEOMYCIN
BODY
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ENDOTHELIUM
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPES
LUNGS
MAMMALS
METASTASES
MICE
NEOPLASMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SARCOMAS
TISSUES
TUMOR CELLS
URACILS
VERTEBRATES