Vascular injury: quantification of experimental focal endothelial denudation in rats using indium-111-labeled platelets

Journal Article · Thu May 01 04:00:00 EDT 1986 · Arteriosclerosis (Dallas); (United States)

DOI:https://doi.org/10.1161/01.ATV.6.3.305· OSTI ID:5687873

Reidy, M A; Yoshida, K; Harker, L A; Schwartz, S M

Rat platelets were labeled with indium-111 (/sup 111/In) and injected into rats whose aortas were subjected to endothelial denudation. A plot of /sup 111/In activity vs the number of missing endothelial cells showed a direct correlation between these parameters and it was possible to detect endothelial denudation when approximately 600 cells were removed. These studies also revealed that intact adjacent endothelium from aortas subjected to injury had detectably increased /sup 111/In activity compared with endothelium of control, uninjured rats. This activity was not found if /sup 111/In-platelets were preincubated with acetylsalicylic acid (1.3 mM) prior to injection and subsequent denudation procedure. To test if /sup 111/In platelet activity could be transported to sites distant from the site of injury, a small catheter (PE 50) was passed down the carotid artery so that it reached the aortic arch or inserted via a femoral artery to the iliac-aortic bifurcation. Significant increases in 111In activity were detected in the lower thoracic aorta when the catheter was introduced into the aortic arch but not when the catheter was introduced via the femoral artery. Morphologic evaluation of these sites showed no endothelial cell loss or adhering platelets. These studies suggest that detectable uptake of released platelet products may occur at distal sites of intact endothelium.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 5687873
• Journal Information: Arteriosclerosis (Dallas); (United States), Journal Name: Arteriosclerosis (Dallas); (United States) Vol. 3; ISSN ARTRD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
INDIUM 111
INDIUM ISOTOPES
INJURIES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RATS
RODENTS
TISSUES
VERTEBRATES