Responsiveness of mouse calvaria to parathyroid hormone after explant cryopreservation: 45Ca release in vitro

Journal Article · Fri Aug 01 00:00:00 EDT 1986 · Cryobiology; (United States)

DOI:https://doi.org/10.1016/0011-2240(86)90039-8· OSTI ID:5003482

Wezeman, F H; Dungan, D D

Newborn mouse calvaria prelabeled with /sup 45/Ca and cryopreserved at -196 degrees C in serum-free medium containing dimethylsulfoxide were compared to unpreserved explants for response to parathyroid hormone during subsequent culture. After short-term cryopreservation followed by rapid thawing, the viable explants continued to release /sup 45/Ca to the culture medium but additions of parathyroid hormone to the medium did not cause increased bone resorption. The data suggest that cryopreservation and thawing impairs mechanisms responsible for parathyroid hormone action on bone cells.

• Research Organization: Loyola Univ. of Chicago, Maywood, IL
• OSTI ID: 5003482
• Journal Information: Cryobiology; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BONE CELLS
CRYOBIOLOGY
CALCIUM 45
FREEZING
MICE
PARATHORMONE
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CALCIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
560201* - Thermal Effects- Cells- (-1987)
551001 - Physiological Systems- Tracer Techniques