Some Effects of Parathyroid Hormone on the Metabolism of Sr ⁸⁵ and Ca ⁴⁵ by Embryonic Chick Bone Cultured in Vitro

Journal Article · Fri Mar 01 04:00:00 EST 1963 · Endocrinology

DOI:https://doi.org/10.1210/endo-72-3-364· OSTI ID:4758495

LENGEMANN, F. W.

Embryonic chick bone was cultured in vitro in a synthetic medium (N.C.T.C.-109) to test the effect of parathyroid extract (PTE) on the metabolism of Ca⁴⁵ and Sr⁸⁵. When contrasted with the control bones, PTE at levels of lation of Sr⁸⁵ and Ca⁴⁵ in the bone and increased Observed Ratio (bone/medium). The low levels of PTE increased, while the very high levels of PTE decreased, both the growth and mass of the bones. The short term uptake of Sr⁸⁵ and Ca⁴⁵ was unaffected by pretreatment of the bone with PTE except at a level of 10 units/ml. PTE at 0.1 and 1.0 unit/ml did not change the proportion of labile to nonlabile Sr⁸⁵ and Ca⁴⁵ in the bones. The nonlabile fraction could be readily mobilized by the addition of PTE into the medium, with 0.1 and 1.0 unit/ml having an apparently equal effect. Sustained PTE treatment of the bones increased the mobility of the nonlabile fraction over that observed after a relatively short exposure to the hormone. PTE acted to reduce the isotope content of growing bone by increasing the movement of mineral from bone to medium, and thus reduced the size of the mineral compartment.

• Research Organization: Cornell Univ., Ithaca, N.Y.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-013933
• OSTI ID: 4758495
• Journal Information: Endocrinology, Journal Name: Endocrinology Journal Issue: 3 Vol. 72; ISSN 0013-7227
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIRDS
BONES
CALCIUM 45
CHICKENS
EMBRYOS
HORMONES
METABOLISM
QUANTITY RATIO
STRONTIUM 85