Responsiveness of mouse calvaria to parathyroid hormone after explant cryopreservation: 45Ca release in vitro
Journal Article
·
· Cryobiology; (United States)
Newborn mouse calvaria prelabeled with /sup 45/Ca and cryopreserved at -196 degrees C in serum-free medium containing dimethylsulfoxide were compared to unpreserved explants for response to parathyroid hormone during subsequent culture. After short-term cryopreservation followed by rapid thawing, the viable explants continued to release /sup 45/Ca to the culture medium but additions of parathyroid hormone to the medium did not cause increased bone resorption. The data suggest that cryopreservation and thawing impairs mechanisms responsible for parathyroid hormone action on bone cells.
- Research Organization:
- Loyola Univ. of Chicago, Maywood, IL
- OSTI ID:
- 5003482
- Journal Information:
- Cryobiology; (United States), Journal Name: Cryobiology; (United States) Vol. 4; ISSN CRYBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001 -- Physiological Systems-- Tracer Techniques
560201* -- Thermal Effects-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BONE CELLS
CALCIUM 45
CALCIUM ISOTOPES
CONNECTIVE TISSUE CELLS
CRYOBIOLOGY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FREEZING
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
NUCLEI
PARATHORMONE
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
560201* -- Thermal Effects-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BONE CELLS
CALCIUM 45
CALCIUM ISOTOPES
CONNECTIVE TISSUE CELLS
CRYOBIOLOGY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FREEZING
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
NUCLEI
PARATHORMONE
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES