Detection of occupational and environmental exposures by bacterial mutagenesis assays of human body fluids
Assays of human body fluids provide a means to document human exposure to mutagens in the environment. In contrast to measurements of ambient levels, these assays demonstrate absorption of mutagens and provide estimates of minimal systemic doses. For most studies reviewed here, specimens of urine were concentrated by adsorption to columns of XAD-2 resin or by liquid partition extraction prior to the mutagenesis assays. The resulting extracts most commonly were analyzed for mutagenicity using the Salmonella/mammalian microsomal plate assay. Less frequently used assays included bacterial fluctuation tests instead of the plate assay and assays for the induction of sister chromatid exchanges in cultured mammalian cells. In addition to reviewing literature reports where body fluids were tested, the advantages, disadvantages, and potential role of this approach will be briefly discussed and compared with other approaches to the identification of mutagenic hazards in the workplace.
- Research Organization:
- National Institute of Environmental Health Sciences, Triangle Park, NC
- OSTI ID:
- 5002801
- Journal Information:
- JOM, J. Occup. Med.; (United States), Journal Name: JOM, J. Occup. Med.; (United States) Vol. 8; ISSN JJOMD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560306 -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL INDICATORS
BIOLOGICAL MATERIALS
BODY FLUIDS
ENVIRONMENTAL EXPOSURE
MATERIALS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENS
SALMONELLA
SCREENING