An experimental analysis of the specificity of actively acquired tolerance in mice

Conference · Thu Aug 15 04:00:00 EDT 1963

OSTI ID:4877708

Doria, G.

Tolerance to CBA skin was induced in C3H mice by neonatal injection of CBA spleen cells. When two months old, the C3H recipients were grafted with CBA skin. These skin grafts showed no signs of rejection during the observation time of three months, whereas CBA skins grafted onto C3H mice non injected at birth showed complete necrosis in 11.7 days. Normal C3H and C3H mice tolerant to CBA skin were injected with rat RBC and sacrificed 12 days later for serum titration of anti-rat RBC agglutinins. The agglutinin titer was the same in both groups. This indicates that the unresponsiveness of C3H mice to CBA skin was specific, for the tolerant mice were able to respond with normal vigor to antigens (rat RBC) unrelated to C3H and CBA. Whether this response was due to the host immune system or to the CBA spleen cells which may have colonized the C3H newborns was subsequently investigated. Spleen cells from tolerant C3H mice sensitized to rat RBC were injected into two groups of lethally irradiated recipients: C3H mice preimmunized against CBA and CBA mice preimmunized against C3H. Both groups were given rat RBC immediately after the spleen cell transfer from the tolerant mice and sacrified a week later for serum titration of anti-rat RBC agglutinins. These agglutinins, due to the secondary response of the transferred spleen cells, could be detected only in the group of C3H recipients preimmunized against CBA. This shows that anti-rat RBC agglutinins in tolerant mice were produced y the immune system of the C3H host. The theoretical implications of this finding are discussed. (auth)

• Research Organization: Euratom, Geneva (Switzerland)
• NSA Number: NSA-18-001367
• OSTI ID: 4877708
• Report Number(s): CONF-239-1; EUR--361-e
• Country of Publication: Switzerland
• Language: English

Related Subjects

ABSORPTION
ACIDITY
AGE
AGGLUTININS
ANNEALING
ANTIBODIES
ANTIGENS
BIOLOGY AND MEDICINE
BLOOD SERUM
COLOR
CONFERENCE
CRYSTALS
CYTOLOGY
DEFECTS
DENSITY
DETECTION
ELECTRONS
HYDROXIDES
IMMUNITY
IRRADIATION
LATTICES
MEASURED VALUES
MICE
NECROSIS
NUMERICALS
OPTICAL SYSTEMS
POTASSIUM CHLORIDES
RATS
SALTS
SKIN
SPLEEN
SUSPENSIONS
TRANSPLANTS
VARIATIONS
X RADIATION