HISTOCHEMICAL STUDIES OF RAT KIDNEY AFTER ABDOMINAL X-IRRADIATION
Journal Article
·
· A.M.A. Arch. Pathol.
OSTI ID:4837010
Enzyme histochemical methods were applied to the study of the x irradiated rat kidney at several dose levels and up to 227 days after irradiation. Tetrazolium formazan staining methods were used for the study of succinate, lactate, malate, and isocitrate dehydrogenase, and triphosphopyridine and diphosphopyidine nucleotide diaphorase. Lead and cobalt sulfide precipitation techniques were used for the study of adenosine triphosphatase, and adenosine-5-, acid, alkaline, and glucose-6-phosphatase. The earliest change noted was a modification in the manner of formazan precipitation in tubule cells. The kidneys of irradiated aninmals showed accumulations of coarse formazan particles. These coarse granules appeared in tubule cells and persisted in both apparently intact and atrophic tubules months after irradiation. Decreased alkaline, acid, and glucose-6phosphatase activity in tubule cells was first noted 3 weeks after irradiation with a total of 4200 r in 7 weekly doses. The decrease in acid phosphatase was accompanied by a coarsening of the sulfide precipitate. Changes in adenosine triphosphatase and adenosine-5-phosphatase activity were not present until 18 and 19 weeks after irradiation, respectively. ln general the changes in the hydrogen transfer and phosphatase systems paralleled the parenchymal loss and progressive renal fibrosis which characterized the postirradiation period. lauth)
- Research Organization:
- Univ. of Chicago
- NSA Number:
- NSA-16-000055
- OSTI ID:
- 4837010
- Journal Information:
- A.M.A. Arch. Pathol., Journal Name: A.M.A. Arch. Pathol. Vol. Vol: 72
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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