Abolition of Neonatally-Induced Homograft Tolerance in Mice by Sublethal X-Irradiation

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Plastic and Reconstructive Surgery

DOI:https://doi.org/10.1097/00006534-196204000-00042· OSTI ID:4835330

Fefer, A.; Nossal, G. J. V.

Tolerance of homologous skin in mice was induced by neonatal intraperitoneal injections of varying doses of homologous spleen cells. In two experiments, partial tolerance was induced by a single injection of spleen from (DBA/2 x C57BL/l0)F₁ and (BALB/c x C3H/6)F₁ hybrids into DBA/2 and BALB/c newborns, respectively. In both cases tolerance was completely abrogated by 350 to 450 r of whole-body x irradiation. In two experiments, a high degree of tolerance was induced by using a strain combination sharing the H-2 antigens, i.e., BALB/c spleen cells injected into DBA/2 newborns, and by multiple injections of DBA/2 neonates with spleen cells from (DBA/2 x C57BL/ l0)F₁ hybrids. Three possible explanations for breakdown of tolerance by irradiation are offered as working hypotheses for further experimentation. The massive proliferation which follows x irradiation may have reestablished the cells whose destruction had induced tolerance, the relevant antigen may have been diluted out by the irradiation and the subsequent cellular proliferation, x irradiation may have favored selectively a sensitized residual population of host cells which had been unaffected by the neonatal injection of antigen. Preliminary results consistent with the 3rd hypothesis are presented. A possible clinical implication of the use of x irradiation to maintain unresponsiveness to a homotransplant is mentioned.

• Research Organization: Stanford Medical Center, Palo Alto, Calif.
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-017549
• OSTI ID: 4835330
• Journal Information: Plastic and Reconstructive Surgery, Journal Name: Plastic and Reconstructive Surgery Journal Issue: 4 Vol. 29; ISSN 0032-1052
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
ANTIGENS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BODY
IMMUNITY
MICE
MITOSIS
QUANTITY RATIO
RADIATION DOSES
RADIOSENSITIVITY
SKIN
SPLEEN
TRANSPLANTS
X RADIATION