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THE ROLE OF WATER IN THE RADIATION "AFTER-EFFECT" (in Russian)

Journal Article · · Doklady Akad. Nauk S.S.S.R.
OSTI ID:4834932
; ;
The necessity for the participation of water in the phenomenon of the radiation after-effect was investigated by irradiating pepsin in an acetate- buffered solution with a pH of 4.65 to 4.8, or in anhydrous glycerin with pH adjusted to 4.6 by acidifying with glacial acetic acid. The damage to the protein was determined by the decrease in proteolytic activity on hemoglobin. Damage to irradiated, dry pepsin (5 to 11 million r dose) can also be detected by thermoinactivation of the pepsin at l00 to 150 deg C in solution. The damaged pepsin is inactivated more rapidly than the intact pepsin in aqueous solution, but the rate of thermoinactivation is the same under anhydrous conditions. On incubating pepsin in aqueous solution at 47 to 54 deg C, it was found that the extent of the thermal after-effect depended only on the irradiation dose, and did not depend on the time or preliminary temperature of the irradiated pepsin. Water is a necessary component for the appearance of the thermal after-effect which is caused by radiation damage. The after-effect does not show itself in the absence of water. (TTT)
Research Organization:
Inst. of Biological Physics, Academy of Sciences, SSSR
NSA Number:
NSA-16-001518
OSTI ID:
4834932
Journal Information:
Doklady Akad. Nauk S.S.S.R., Journal Name: Doklady Akad. Nauk S.S.S.R. Vol. Vol: 140
Country of Publication:
Country unknown/Code not available
Language:
Russian

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Related Subjects

ACETIC ACID
BIOLOGY AND MEDICINE
ENZYMES
HEMOGLOBIN
PEPSIN
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
TEMPERATURE
WATER