A STUDY OF THE MECHANISM OF RADIATION "AFTER EFFECT" IN PROTEINS
Journal Article
·
· Biophysics (U.S.S.R.) (English Translation)
OSTI ID:4190400
The phenomenon of radiation after effect was observed following irradiation of solutions of myosin with ionizing radiations. The magnitude of after effect in the fraction of the protein molecules which retain their activity after irradiation was proportional to the degree of direct inactivation of the enzyme during irradiation. Study of the kinetics of thermal inactivation of irradiated solutions of myosin indicated that the after effect reaction was intramolecular in character. The activation energy of after effect (postradiation thermal indativation) in irradiated myosin was markedly different from the activation energy of thermal inactivation of unirradiated protein (77 kcal/ mole) and was not dependent on dose. Analysis of published data on radiation after effect in trypsin, pepsin, and egg albunain indicates the similarity of the mechanism of radiation after effect in different proteins. Different possible explanations of the mechanism of radiation after effect in proteins have been analyzed on the basis of the experimental results obtained. The experimertal results are in agreement with a theory involving induction of special persistent excited states of the protein molecule during irradiation, characterized by a low activation energy of thermal inactivation. A hypothesis is put forward concerning the possible role of macromolecular ordered structure in water in the mechanism of radiation inactivation of enzymes. (auth)
- Research Organization:
- Inst. of Biological Physics, Academy of Sciences, Moscow
- NSA Number:
- NSA-14-009339
- OSTI ID:
- 4190400
- Journal Information:
- Biophysics (U.S.S.R.) (English Translation), Journal Name: Biophysics (U.S.S.R.) (English Translation) Vol. Vol: 4
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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