THE OXYGEN EFFECT IN IRRADIATION OF DORMANT BARLEY SEEDS

Journal Article · Fri Sep 01 04:00:00 EDT 1961 · Izvest. akad. Nauk S.S.S.R., Ser. Biol.

OSTI ID:4831174

Nuzhdin, N I; Dozortseva, R L

Barley seeds of two stralns, viz. the Moscow and Odessa 17, received 12,000 r of gamma rays from Co/sup 60/. The seeds of the former strain were irradiated in air in the state of dormancy. Immediately after irradiation they were divided into three groups, and for further storage one group was transferred into N, the second in O/sub 2/, and the third one stored in air. The seeds of the Odessa-17 strain were irradiated in the dormant and resting state. Part of the seeds were irradiated in N and others in O/sub 2/. After irradiation the seeds were stored in the atmosphere of the same gases in which irradiation was performed. The radiation effect was estimated from the frequency of cells with chromosome bridges in ana- and telophase of the first mitoses in the meristeme of root tips. The effect was similar with seeds irradiated in air andd then kept in N or irradiated in this gas. Anoxia greatly reduced the percent of cells with chromosome bridges no matter whether the seeds were irradiated in the dormant or resting condition. Unlike the results obtained in the N series, two kinds of result were obtained upon irradiation and storage of the irradiated seeds in O/ sub 2/ or alr. The percentage of cells with chromosome bridges was high both in seeds irradiated in the dormant or resting condition when dormant seeds after spontaneous or artificial release from this condition were stored, prior to germination, in oxygen or in air. In seeds irradiated in the state of dormancy, artificially released from this condition and immediately germinated, the percent of cells with bridges is similar to that of seeds irradiated in N. Hence, in seeds irradiated in the dormant state prior to release from this condition the O/ sub 2/ effect is absent. This is presumably linked with reduced metabolism of these seeds. (auth)

• Research Organization: Inst. of Genetics, Academy of Sciences, Moscow
• NSA Number: NSA-16-001533
• OSTI ID: 4831174
• Journal Information: Izvest. akad. Nauk S.S.S.R., Ser. Biol., Journal Name: Izvest. akad. Nauk S.S.S.R., Ser. Biol. Vol. Vol: No. 5
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

AIR
BIOLOGY AND MEDICINE
CEREALS
CHROMOSOMES
COBALT 60
GAMMA RADIATION
METABOLISM
MITOSIS
NITROGEN
OXYGEN
PLANT CELLS
RADIATION DOSES
RADIATION EFFECTS
ROOTS
SEEDS
STORAGE