Preparation of Radiocyanocobalamin-60Co (in Japanese)
Journal Article
·
· Radioisotopes (Tokyo)
When cobalt-60 was added in the fermentation medium of Streptomyces griseus, cyanocobalamin (vitamin B₁₂) labeled with Co⁶⁰ was obtained by biosynthesis. The extraction and purification method of radiocyanocobalamin from the fermentation broth was established in consideration of improving radiochemical purity and protection against radiation. In this procedure radiocyanocobalamin was purifled using the techniques of chromatography with alumina and treatment with ion exchange resins. It was evidenced by paper chromatography that the radioactive impurities were thoroughly removed by this purification procedure. Also, the method of quality test of radiocyanocobalamin solution was established. Finally, about 1 mc of Co⁶⁰ with specific activity of 14 mc/mg was added in the fermentation medium and radiocyanocobalamin- Co⁶⁰ was prepared. In the purified solution, radiocyanocobalamin with specific activity of 0.5 mc/mg was contained with concentration of 1.1 mic ons/ml. This solution was sufficientiy pure for practical
- Research Organization:
- Dainippon Pharmaceutical Co., Ltd., Japan
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-018909
- OSTI ID:
- 4830653
- Journal Information:
- Radioisotopes (Tokyo), Journal Name: Radioisotopes (Tokyo) Journal Issue: 4 Vol. 10; ISSN 0033-8303
- Country of Publication:
- Country unknown/Code not available
- Language:
- Japanese
Similar Records
Stability of Injected Vitamin B12-Co60 and Vitamin B12 Content of Dog Liver
The Preparation of H 3 -Vitamins D 2 and D 3 and Their Localization in the Rat *
Recombinant human lymphotoxin: Expression in Escherichia coli, purification, and some properties
Journal Article
·
Sat Oct 01 00:00:00 EDT 1960
· Experimental Biology and Medicine
·
OSTI ID:4807701
The Preparation of H 3 -Vitamins D 2 and D 3 and Their Localization in the Rat *
Journal Article
·
Sun Sep 01 00:00:00 EDT 1963
· Biochemistry
·
OSTI ID:4634836
Recombinant human lymphotoxin: Expression in Escherichia coli, purification, and some properties
Journal Article
·
Fri Sep 01 00:00:00 EDT 1995
· Biochemistry (New York)
·
OSTI ID:161891