Stability of Injected Vitamin B12-Co60 and Vitamin B12 Content of Dog Liver

Journal Article · Sat Oct 01 04:00:00 EDT 1960 · Experimental Biology and Medicine

DOI:https://doi.org/10.3181/00379727-105-26038· OSTI ID:4807701

Rosenblum, C.; Willigan, D. A.; Meriwether, H. T.; Cronkite, E. P.

An investigation was made of livers from 5 dogs receiving 3.6 to 14 μg (specific activity = 1 mc/mg) of vitamin B₁₂-Co⁶⁰ intravenously. The vitamin B₁₂-Co⁶⁰, was retain ed by the livers as an intact cobalamin, probably as cyanocobalamin. This was demonstrated by the method of reverse isotope dilution. Direct isotope dilution indicated an average total cobalamin concentration of 0.36 μg/g of dog liver. The authenticity of retained vitamin B₁₂-Co⁶⁰, introduced by injection, as an isotope dilution tracer was demonstrated by simultaneous analysis with vitamin B₁₂ Co⁵⁷. Degradation of vitamin B₁₂ to ionic cobalt was negligible.

• Research Organization: Brookhaven National Lab., Upton, N.Y.; Merck Sharp and Dohme Research Labs., Rahway, N.J.
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-023397
• OSTI ID: 4807701
• Report Number(s): BNL-4888
• Journal Information: Experimental Biology and Medicine, Journal Name: Experimental Biology and Medicine Journal Issue: 1 Vol. 105; ISSN 1535-3702
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
COBALT
COBALT 57
COBALT 60
DOGS
IONS
LIVER
MEASURED VALUES
METABOLISM
QUANTITY RATIO
TRACER TECHNIQUES
VITAMIN B-12
VITAMINS