The Preparation of H 3 -Vitamins D 2 and D 3 and Their Localization in the Rat *
A convenient procedure for the preparatioNonen of tritiumlabeled vitamin D/sub 2/ and D/sub 3/ is described. The provitamins were exposed to tritium gas by the procedure of Wilzbach, irradiated with ultraviolet light, and isolated as the crystalline 3,5-dinitrobenzoates. The H/sup 3/-vitamin D/sub 2/, specific activity 3.2 mC/mmole, or 450 disintegrations per minute per IU, and H/sup 3/- vitamin D/sub 3/, specific activity 7.2 mC/mmole or 1050 dpm/IU, are fully biologically active and were shown to be radiochemically pure in at least three chromatographic systems. The distribution of a physiological dose of Ha-vitamin D/sub 3/ as a function of time has been studied in the various tissues of the rat. Evidence is presented for the involvement of the ileum in the absorption of vitamin D. The liver rapidly accumulated the largest amount of radioactivity whether the dose was administered orally or intracardially. No evidence was found for the involvement of the adrenals in the metabolism of vitamin D. (auth)
- Research Organization:
- Univ. of Wisconsin, Madison
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-038710
- OSTI ID:
- 4634836
- Journal Information:
- Biochemistry, Journal Name: Biochemistry Journal Issue: 5 Vol. 2; ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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