REJECTION OF SKIN GRAFTS AND PRODUCTION OF SPECIFIC ISOHEMAGGLUTININS BY NORMAL AND X-IRRADIATED MICE
The immune responses of normal and x-irradiated mice were compared by studying the survival of skin homografts and by following changes of isohemagglutinin titers in host serum. Male CBA mice were presensitized by intraperitoneal injection of spleen cells from strain A mice, and 7 days later were irradiated with 200, 400, or 750 rads, given at a rate of 73 rads/min. They were then grafted with A-strain skin on the 7th, 29th, or 91st day after sensitization. Graft rejection and hemagglutinin formation were hoth depressed by radiation in proportion to the dose given. The radioresistance of the hemagglutinin response was greater in mice sensitized by spleen cells than in nonsensitized mice. The 400-rad dose suppressed this response completely in nonsensitized mice although it only slightly retarded the rejection of 2 successive skin homografts. These results confirm that circulating antibody is not essential for the rejection of skin grafts. They also suggest that plasma cells are not involved in graft rejection and that these cells are most radiosensitive before actively synthesizing antibody, since irradiation one week after presensitization has relatively little effect on hemagglutinin production. (H.H.D.)
- Research Organization:
- Medical Research Council, Harwell, Berks, Eng.
- NSA Number:
- NSA-16-020267
- OSTI ID:
- 4829211
- Journal Information:
- Immunology (England), Vol. Vol: 4; Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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