LONG-TERM CULTIVATION IN TISSUE CULTURE OF LEUKEMIC CELLS FROM MOUSE LEUKEMIA INDUCED BY MOLONEY VIRUS OR BY X RAYS
Journal Article
·
· Journal of the National Cancer Institute (U.S.) Changed to JNCI, J. Natl. Cancer Inst.
OSTI ID:4828872
Successful long-term in vitro cultures of leukemic cells were obtained from BALB/c, C57BL/6, SWR, and Swiss mice, with leukemia induced by Moloney virus; and from C57BL/6 mice, with leukemia induced by x rays. The leukemic cells were grown from 4to 10 months in vitro. All the cell lines were successfully maintained on feeder layers of normal mouse spleen cells in Eagle's medium supplemented with horse serum. Under these conditions the leukemic cells grew free in the medium, so that they could be readily passaged without trypsinization. The leukemic nature of the cultured cells was established by examination of cell smears and by cell transplantation into animals. (auth)
- Research Organization:
- Weizmann Inst. of Science, Rehovoth, Israel
- NSA Number:
- NSA-16-002903
- OSTI ID:
- 4828872
- Journal Information:
- Journal of the National Cancer Institute (U.S.) Changed to JNCI, J. Natl. Cancer Inst., Journal Name: Journal of the National Cancer Institute (U.S.) Changed to JNCI, J. Natl. Cancer Inst. Vol. Vol: 27; ISSN JNCIA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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