Nature of leukemic stem cells in murine myelogenous leukemia

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Int. J. Cell Cloning; (United States)

DOI:https://doi.org/10.1002/stem.5530040203· OSTI ID:5687352

Yoshida, K; Nemoto, K; Nishimura, M; Hayata, I; Inoue, T; Seki, M

We investigated the nature of myelogenous leukemic stem cells in mice. L-8057, a megakaryoblastic leukemia cell line used in this study, produces in vivo and in vitro colonies. By means of typical chromosomal aberrations in L-8057, one can conveniently detect the origin of the cells in each colony derived from a leukemic stem cell. Direct evidence of whether cells from each colony had leukemogenicity in recipient mice was successfully obtained by the colony transplantation assay. Both leukemic colony-forming unit-spleen (L-CFU-s) and leukemic colony-forming unit-culture (L-CFU-c) in L-8057 may have belonged to the same differentiating stage in the stem cells because of their similar radiosensitivity, although some parts of the L-CFU of L-8057 seemed to have lost their capability to regenerate L-CFU-s when the cells were plated in dishes. This leukemic stem cell preserves high self-renewal ability in vitro after 10 passages. In addition, in vitro colony formation by this leukemic cell during the above course of serial passages did not require any additional exogenous stimulators. The same sort of trials have been made on other types of leukemias. Leukemic stem cells showed remarkable variety in their response to stimulating factors and in their self-renewal activity, which suggests that they may have consisted of heterogeneous populations.

• Research Organization: National Institute of Radiological Sciences, Chiba, Japan
• OSTI ID: 5687352
• Journal Information: Int. J. Cell Cloning; (United States), Journal Name: Int. J. Cell Cloning; (United States) Vol. 2; ISSN IJCCE
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOGENESIS
CELL CULTURES
CELL DIFFERENTIATION
CHROMOSOMAL ABERRATIONS
COLONY FORMATION
DISEASES
ERYTHROCYTES
HEMIC DISEASES
LEUKEMIA
LEUKEMOGENESIS
LEUKOCYTES
MAMMALS
MATERIALS
MICE
MONOCYTES
MUTATIONS
NEOPLASMS
ORGANS
PATHOGENESIS
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
VERTEBRATES