Phenotypic and genotypic characterization of radiation sensitivity in Escherichia coli B

Journal Article · Mon Aug 20 04:00:00 EDT 1962 · Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

DOI:https://doi.org/10.1016/0926-6550(62)90090-7· OSTI ID:4821882

Rörsch, A.; Edelman, A.; Van Der Kamp, C.; Cohen, J. A.

From the wild type Echerichia coli B a radioresistant and a radiosensitive mutant were isolated. Ultraviolet light survival curves for the newly isolated strains resemble those given for E. coli B/r and E. coli B respectively. It was proved by biochemical methods that the differences among the three strains are due to two independent mutations, one leading to loss of the tendency to form filaments after various treatments and the other leading to a strong inhibition of growth, nucleic acid, and protein synthesis after irradiation with uitraviolet light. X-ray, ultraviolet light, P decay, and nitrous acid survival curves for the strains are presented. Moreover, survival curves are given for a fourth strain which bears both independent mutations. Procedures are described for screening large numbers of strains arising from conjugation experiments for both types of mutation. Conjugation experiments between F/sup +/ and F/sup -/ auxotrophic mutants were performed. These suggest that both mutations are chromosomally determined. (auth)

• Research Organization: National Defence Research Organization TNO, Rijswijk, Netherlands
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-026775
• OSTI ID: 4821882
• Journal Information: Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, Journal Name: Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Journal Issue: 2 Vol. 61; ISSN 0926-6550
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACIDS
BACTERIA
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CHROMOSOMES
DECAY
ESCHERICHIA COLI
GENETICS
MINERAL ACIDS
MUTATIONS
NITROUS ACID
NUCLEIC ACIDS
PHOSPHORUS
PROTEINS
RADIOSENSITIVITY
ULTRAVIOLET RADIATION
X RADIATION