THE GENE-CONTROLLED RADIATION SENSITIVITY IN ESCHERICHIA COLI

Journal Article · Tue Feb 26 04:00:00 EST 1963 · Biochimica et Biophysica Acta (Netherlands)

DOI:https://doi.org/10.1016/0006-3002(63)90141-0· OSTI ID:4728060

Roersch, A; Edelman, A; Cohen, J A

The position of the genetic determinant syn on the chromosome of Escherichia coli strain B was determined by conjugation experiments. This syn marker, which affects the radiation sensitivity of the strain, is located between the markers controlling xylose fermentation and streptomycin resistance. The fertility factor of E. coli K 12 strain was transferred to an auxotrophe of a radiation-sensitive mutant of E. coli B (syn-). By subjecting the latter to conjugation with E. coli C the syn- marker was transferred to that strain. Besides its increased ultraviolet-light sensitivity a syn- strain differs from the wild type (syn/sup +/) by its decreased ability to propagate ultraviolet- inactivated bacteriophage T/sub 1/, T/sub 3/, T/sub 7/ and No measurable . It is concluded that like the u gene of bacteriophage T/sub 4/, the bacterial syn gene controls a process, concerned with the repair of ultraviolet-light lesions. (auth)

• Research Organization: Rijksverdedigingsorganisatie, TNO, Rijswijk, Netherlands
• NSA Number: NSA-17-021491
• OSTI ID: 4728060
• Journal Information: Biochimica et Biophysica Acta (Netherlands), Journal Name: Biochimica et Biophysica Acta (Netherlands) Vol. Vol: 68; ISSN BBACA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BACTERIA
BACTERIOPHAGES
BIOLOGY AND MEDICINE
CHROMOSOMES
ESCHERICHIA COLI
GENETICS
RADIATION EFFECTS
RADIOSENSITIVITY
ULTRAVIOLET RADIATION