STUDIES ON LYMPHOCYTES. I. LYMPHOPENIA PRODUCED BY PROLONGED EXTRACORPOREAL IRRADIATION OF CIRCULATING BLOOD

Journal Article · Wed Aug 01 04:00:00 EDT 1962 · Blood (U.S.)

OSTI ID:4820729

Cronkite, E P; Jansen, C R; Mather, G C; Nielsen, N O; Usenik, E A; Adamik, E R; Sipe, C R

A method for extracorporeal irradiation of the circulating blood is described. Extracorporeal irradiation of the blood will produce a lymphopenia promptly which persists for weeks. Heparin in high doses in the calf produces a lymphocytosis and neutrophilic leukocytosis. (auth) Red cell sensitization by either agglutinating or incomplete antibodies was detected with an I/sup 131/ labeled rabbit antihuman globulin serum (RAG). Non-specific absorption of RAG by red cells was reduced to a minimum by the addition of 6% bovine albumin. The reactions between RAG and the sensitized erythrocytes were typical of antigen- antibody reactions and the sensitivity of the test was found to be greater than the standard Coombs test. Quantitative studies of the degree of erythrocyte sensitization by isoimmune or autoimmune antibodies were possible with this technic. (auth)

• Research Organization: Brookhaven National Lab., Upton, N.Y.
• NSA Number: NSA-16-023434
• OSTI ID: 4820729
• Report Number(s): BNL-6011; 0006-4971
• Journal Information: Blood (U.S.), Journal Name: Blood (U.S.) Vol. Vol: 20; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BLOOD
BODY
CATTLE
DRUGS
HEPARIN
IRRADIATION
LYMPHOCYTES
QUANTITY RATIO
RADIATION EFFECTS