RNA as a Protective Agent Against Irradiation of Cell Cultures

Journal Article · Wed Nov 01 04:00:00 EST 1961 · Annals of the New York Academy of Sciences

DOI:https://doi.org/10.1111/j.1749-6632.1961.tb50094.x· OSTI ID:4809676

Rounds, Donald E.

The Fernandes strain of amnion cells, treated with dosages ranging from 0.01 to 0.5 mg/ml of yeast RNA 24 hours prior to 700 r gamma radiation, showed increased survival as compared with control cultures not so treated. Approximately 3 times as many cells survived 700 r when pretreated with 0.2 mg/ml yeast RNA. A minimum RNA treatment time of 8 to 10 hr was necessary before a protective response could be elicited in irradiated amnion cells. Thus protection may result only after the yeast RNA is utilized by the cell. RNA- treated and control cultures of amnion cells were exposed to gamma doses ranging from 0 to 700 r. Survival was represented by parallel lines on a semilogarithmic plot, which suggested a possible increase in the cell number of treated cultures prior to the onset of radiation damage. Daily counts of amnion cells indicated that RNA-treated elements multiply at a normal rate for 2 days postirradiation, whereas untreated irradiated cultures showed an immediate inhibition in the rate of growth. Seven different established cell strains exhibited variations in the response of preirradiation treatment with yeast RNA. Four cell types of nonmalignant origin showed from 0.5 to 8 times more cells surviving 700 r when pretreated with RNA than untreated, irradiated cultures. Three cell lines of malignant origin showed no evidence of protection.

• Research Organization: Pasadena Foundation for Medical Research, Calif.
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-026767
• OSTI ID: 4809676
• Journal Information: Annals of the New York Academy of Sciences, Journal Name: Annals of the New York Academy of Sciences Journal Issue: 2 Vol. 95; ISSN 0077-8923
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMNION CELLS
ANIMAL CELLS
BIOLOGY AND MEDICINE
CANCER
GAMMA RADIATION
RADIATION DOSES
RADIATION INJURIES
RADIATION PROTECTION
RADIOSENSITIVITY
RIBONUCLEIC ACID