RADIOPROTECTIVE AGENTS. THE EFFECTS OF DEPOLYMERIZED YEAST RIBONUCLEIC ACID ON RADIATED HUMAN CELLS IN TISSUE CULTURE

Technical Report · Mon Oct 01 04:00:00 EDT 1962

OSTI ID:4727744

Rounds, D E; Slick, W C

Yeast ribonucleic acid was hydrolyzed with NaOH to produce polynucleotide mixtures which were tested for radioprotective activity on cultured cells. The depolymerized RNA stimulated the growth rate of amnion cells during 5-day treatment periods, but had no effect on growth when the cells were treated for a 24-hour period. A 24hour treatment of amnlon cells with the nucleotide mixture prior to 700 r gamma radiation resulted in an increase in the surviving cell populations. This effect appeared to reach a maximum with a polynucleotide concentration of 0.3 mg/ml. The RNA hydrolysate had no significant effect on radiated KB cells, but surviving HeLa and amnion cell populations were increased by 19% and 63%, respectively, over controls. This radioprotective response was detected after a minimum of 4 hours of treatment of amnion cells with the test material. (auth)

• Research Organization: Pasadena Foundation for Medical Research, Calif.
• NSA Number: NSA-17-015803
• OSTI ID: 4727744
• Report Number(s): SAM-TDR-62-85
• Country of Publication: United States
• Language: English

Related Subjects

AMNION CELLS
ANIMAL CELLS
BIOLOGY AND MEDICINE
EFFICIENCY
ENVIRONMENT
EXPANSION
GAMMA RADIATION
HELA CELLS
HYDROLYSIS
MAN
NUCLEIC ACIDS
NUCLEOTIDES
PREPARATION
RADIATION DOSES
RADIATION PROTECTION
RIBONUCLEIC ACID
SEPARATION PROCESSES
SODIUM HYDROXIDES
SURVIVAL TIME
TESTING
TISSUE CULTURES
TISSUES
YEASTS