DNA-synthesis in Nuclei Isolated from Regenerating Rat Liver after Whole-body Irradiation

Journal Article · Wed Aug 01 04:00:00 EDT 1962 · International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine

DOI:https://doi.org/10.1080/09553006214550891· OSTI ID:4797779

Lehnert, S. M.; Okada, S.

Incorporation of tritiated thymidine into DNA of nuclei isolated from the regenerating livers of rats given 800 r of whole-body radiation 24 hours after partial hepatectctomy has been studied in the presence of a DNA- synthesizizing enzyme system. Immediately after irradiation no diffeference in incorporation was demonstrable between irradiatated and non-irradiated liver nuclei, With increasing time after irradiation, up to 24 hours, incorporation became progressively depressed. At 24 hours after irradiation, incorporation was depressed to the same extent after doses of 200 to 3000 r. Incorporation into nuclei of liver from nonhepatectomized rats was unaffected 24 hours after a dose of 800 r. In contrast to the findings with isolated nuclei, DNA extracted from irradiated regenerating liver, 24 hours after a dose of 800 r, incorporated tritiated thymidine to the same extent as did DNA extracted from non-irradiated regenerating liver. Mechanisms by means to which these findings may be explained are discussed. (auth)

• Research Organization: Univ. of Rochester, N.Y.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-000075
• OSTI ID: 4797779
• Journal Information: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, Journal Name: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine Journal Issue: 4 Vol. 5; ISSN 0020-7616
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMINO ACIDS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BODY
DNA
ENZYMES
IRRADIATION
LABELLED COMPOUNDS
LIVER
NUCLEIC ACIDS
RADIATION DOSES
RATS
REGENERATION
RIBONUCLEIC ACID
SURGERY
THYMIDINE
TRACER TECHNIQUES
TRITIUM