THE EFFECTS OF CHRONIC IRRADIATION ON DNA SYNTHESIS IN REGENERATING MOUSE LIVER

Journal Article · Sat Oct 01 04:00:00 EDT 1960 · Cancer Research

OSTI ID:4136870

Lesher, S; Stroud, A N; Brues, A M

The rate at which liver cells synthesize desoxyribonucleic acid (DNA) and divide following partial hepatectomy has been studied in LAF/sub 1/ mice exposed to 12 r per day of CoQ gamma irradiation for 350 days starting at 100 days of age and two nonirradiated groups of 120 and 450 days of age. The amount of DNA per individual nucleus was determined photometrically in Feulgen-stained preparations. The incorporation of tritiated thymidine into parenchymal liver nuclei at various time intervals after partial hepatectomy was used as a measure of DNA synthesis. DNA synthesis and cell division decrease with age and are further decreased by extended exposure to daily low-dose gamma irradiation. The data suggest that although chrontc irradiation delays the onset of DNA synthesis to some extent it inhibits it only to a limited degree. It also interferes with cell division, possibly owing to accumulated chromosome damage; increased ploidy of liver cells in the previously irradiated animals may be accounted for by this. (auth)

• Research Organization: Argonne National Lab., Ill.
• NSA Number: NSA-14-025313
• OSTI ID: 4136870
• Journal Information: Cancer Research, Journal Name: Cancer Research Vol. Vol: 20
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
AMINO ACIDS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CHROMOSOMES
COBALT 60
DNA
GAMMA RADIATION
LABELLED COMPOUNDS
LIVER
MITOSIS
NUCLEIC ACIDS
RADIATION DOSES
RADIATION INJURIES
REGENERATION
THYMIDINE
TRITIUM