Radiosensitivity of resting and proliferating mouse-liver cells for mitotic delay and the inhibition of DNA synthesis

Journal Article · Fri Feb 01 04:00:00 EDT 1974 · Int. J. Radiat. Biol., v. 25, no. 2, pp. 197-203

DOI:https://doi.org/10.1080/09553007414550221· OSTI ID:4310667

Coggle, J E

Groups of 75 mice were given 450 rads whole-body 250 kVp x rays either 4 hr before, 12 hr after, or 32 hr alter partial hepatectomy i.e., when most of the liver cells were in the G/sub o/ phase, G phase, and S phase respectively. All groups showed a delay in the onset of division and a reduction in total mitotic activity. Similar groups of 75 were given the same radiation dose at the same timepoints after surgery, then killed in groups of 15, 1 hr before which they were given intraperitioneally 1 mu Ci g/sup -1/ body-weight of tritiated thymidine. All irradiated groups showed a marked delay in the onset of /sup 3/H- labeling. noue having occurred 36 hr after operation. The irradiated groups also showed a marked reduction in the total number of cells undergoing DNA synthesis, with the exception of cells irradiated when they were regenerating and in DNA synthesis i.e., about 32 hr after partial hepatectomy. (UK)

• Research Organization: Saint Bartholomew's Hospital, London
• NSA Number: NSA-29-029825
• OSTI ID: 4310667
• Journal Information: Int. J. Radiat. Biol., v. 25, no. 2, pp. 197-203, Journal Name: Int. J. Radiat. Biol., v. 25, no. 2, pp. 197-203; ISSN IJRBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

CELL CYCLE
CELL PROLIFERATION
LIVER
MICE
MITOSIS
MITOTIC DELAY
N48140 --Life Sciences--Radiation Effects on Biochemicals--In Animals
N48210 --Life Sciences--Radiation Effects on Cells--External Source
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
RADIOSENSITIVITY
RESPONSE MODIFYING FACTORS
THYMIDINE
TIME DEPENDENCE
TRITIUM COMPOUNDS
WHOLE-BODY IRRADIATION
X RADIATION