THE SIGNIFICANCE OF FAT RESERVES IN THE CELL IN THE REACTIVATION OF THE CELL AFTER RADIATION DAMAGE (in Russian)
Yeast cells (Endomyces magnusii and Saccharomyces vini, Megri strains 139-B) were subjected to an x-ray dose of 150 and 250 kr. Cells containing 11.5% fat were grown by increasing the sugar content and decreasing the content of glycolic acid and phosphorus in the growth medium. Cells with a low fat content of 2.2% were also subjected to radiation. The fat-rich cells of Endomyces magnasii were more resistant to radiation than the fat-poor cells, as determined by the number of viable cells grown on an agar-wort medium (28% survivals as compared to 9.6% for the fat-poor cellsl. Significant recuperation of the fat-rich cells was noted in 24 hours, but the fat-poor cells had shown hardly any recovered. Maximum reactivation (78.5%) of the fatrich cells was noted on the 4th day, while the fat-poor cells showed maximum reactivation (62%) on the 5th day. With a dose of 250 kr the comparative figures are 26.4 and 13.6% respectively. Examination of the cells by luminescent microscopy with Nile blue (1: 40,000) showed that the fat reserves are utilized by the cell in the process of recuperation. Yeast cells of Saccharomycetes vini, Megri 139-B strain, gave analogous results. (TTT)
- Research Organization:
- Inst. of Microbiology, Academy of Sciences, USSR; Inst. of Biological Physics, Academy of Sciences, USSR
- NSA Number:
- NSA-16-014495
- OSTI ID:
- 4785306
- Journal Information:
- Doklady Akad. Nauk S.S.S.R., Journal Name: Doklady Akad. Nauk S.S.S.R. Vol. Vol: 142
- Country of Publication:
- Country unknown/Code not available
- Language:
- Russian
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