SOME ASPECTS OF THE STRUCTURE OF HEAT DENATURED AND ULTRAVIOLET IRRADIATED DNA

Journal Article · Wed Nov 01 04:00:00 EST 1961 · Journal de Chimie Physique (France) Merged with Rev. Gen. Colloides to form J. Chim. Phys. Rev. Gen. Colloides

OSTI ID:4785251

Grossman, L; Stollar, D; Herrington, K

DNA, denatured by heat and rapidly cooled, possesses a structure in which bending occurs at random. The hydrogen bonds between the amine groups of the purine and pyrimidine bases impose a specific secondary structure. The peculiarities of the secondary structure of DNA denatured by heat were studied by means of the reaction with formaldehyde followed by spectrophotometric, immunologic, and enzymologic methods. The utilization of these techniques, together with CsCl density gradient analysis, showed that the uv irradiation of DNA in solution produces an irreversible lesion of the hydrogen bonding, most probably in the internal regions of the DNA molecule, and the formation of formamidic bonds and of thermostable bonds between the two complementary chains of DNA. The separation of this last lesion is catalyzed by the photoreactivator enzyme of baker's yeast. (tr-auth)

• Research Organization: Brandeis Univ., Waltham, Mass.
• NSA Number: NSA-16-014524
• OSTI ID: 4785251
• Journal Information: Journal de Chimie Physique (France) Merged with Rev. Gen. Colloides to form J. Chim. Phys. Rev. Gen. Colloides, Journal Name: Journal de Chimie Physique (France) Merged with Rev. Gen. Colloides to form J. Chim. Phys. Rev. Gen. Colloides Vol. Vol: 58; ISSN JCPQA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALDEHYDES
AMINES
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CESIUM CHLORIDES
DENSITY
ENZYMES
FORMALDEHYDE
FORMIC ACID
IMMUNITY
NUCLEIC ACIDS
PHOTOMETRY
PHOTOREACTIVATION
RADIATION EFFECTS
SOLUTIONS
SPECTRA
TEMPERATURE
ULTRAVIOLET RADIATION