UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice

Journal Article · Sun Jun 01 04:00:00 EDT 1997 · Environmental and Molecular Mutagenesis

DOI:https://doi.org/10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C· OSTI ID:478377

Frijhoff, A F.W.; Rebel, H; Mientjes, E J ^[1]

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1. TNO Nutrition and Food Research Institute, Zeist (Netherlands); and others

UVB-induced mutagenesis was studied in hairless 40.6 transgenic mice (Muta{trademark}Mouse), which contain the {lambda}gt1OlacZ shuttle vector as a target for mutagenesis. Mice were exposed at the dorsal side to either single doses of 200, 500, 800, or 1000 J/m{sup 2} UVB or to two successive irradiations of either 200 and 800 J/m{sup 2} UVB, with intervals of 1,3, or 5 days, or to 800 and 200 J/m{sup 2} UVB with a 5-day interval. At 23 days after the last exposure, lacZ mutant frequencies (MF) were determined in the epidermis. The lacZ MF increased linearly with increasing dose of UVB. The mutagenic effect of two successive irradiations appeared to be additive. The UV-induced mutation spectrum was dominated by G:C{r_arrow}A:T transitions at dipyrimidine sites. DNA-sequence analysis of spontaneously mutated phages showed a diverse spectrum consisting of insertions, deletions and G:C {r_arrow} A:T transitions at CpG sites. the results indicate that the hairless {lambda}lacZ-transgenic mouse is a suitable in vivo model for studying UVB-induced mutations. 29 refs., 5 tabs.

• OSTI ID: 478377
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: 2 Vol. 29; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
GENETIC RADIATION EFFECTS
MICE
MUTATIONS
ULTRAVIOLET RADIATION