Some Observations on Irradiation Effects in Yeast
Two strains of yeast, a haploid and a diploid one, were irradiated with x rays, and survival curves were obtained by colony counts. In haploid yeast, the viability decreased exponentially with increasing x-ray dose, whereas the diploid strain exhibited a plateau in the dose-response curve similar to that previously described by other workers for haploid yeast. The number of survivals and the shape of the survival curves varied with the physiological condition of the cells at the moment of irradiation. Thus, for example, in baker's yeast, deprivation of a source of nitrogen prior to irradiation resulted in a certain degree of protection, when compared with the survival of fresh nonstarved cells. Considerable protection was further afforded in both strains of yeast if cells were grown and irradiated in high concentration of substrate (55% fructose). Lastly, nitrogen deprivation interposed between irradiation and plating also favored recovery. Reasons are suggested for these changes in support of the view that indirect effects of irradiation contribute to a large extent to loss of viability in yeast. (auth)
- Research Organization:
- Cambridge Univ., Eng.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-016223
- OSTI ID:
- 4782495
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 4 Vol. 16; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
THE YIELD OF BIOCHEMICAL MUTATION REVERSALS IN YEAST AS A FUNCTION OF THE PLOIDY ON INTERACTION WITH VARIOUS KINDS OF RADIATION
Comparative study of RBE with exposure to alpha radiation of yeast cells at different stages of growth
Induction of dominant lethality by x rays in a radiosensitive strain of yeast
Journal Article
·
Tue Dec 31 23:00:00 EST 1963
· Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals
·
OSTI ID:4090105
Comparative study of RBE with exposure to alpha radiation of yeast cells at different stages of growth
Journal Article
·
Fri Dec 31 23:00:00 EST 1976
· Radiobiology (USSR) (Engl. Transl.); (United States)
·
OSTI ID:7316477
Induction of dominant lethality by x rays in a radiosensitive strain of yeast
Journal Article
·
Mon Oct 01 00:00:00 EDT 1973
· Mutat. Res., v. 20, no. 1, pp. 45-51
·
OSTI ID:4404631