EXPERIMENTAL RADIATION DAMAGE OF THE CEREBELLUM OF THE MOUSE AFTER HIGH DOSAGE ROENTGEN IRRADIATION. (A STUDY WITH NEUROHISTOLOGIC IMPREGNATION PROCEDURES)
The cerebellum was irradiated with a single dose of 10 or 60 kr delivered at a rate of 3.7 kr/min, the rest of the animal being shielded. No gross effects were noted with the low dose but with the high dose the animals died within 6 hr to 5 days. Six to 8 hr after the high dose, the meninges appeared red but no other gross changes of the brain were evident. However, histopathologic changes could be noted as early as 2 hr after 60 kr, especially with Ag and Au impregnation techniques. The earliest change was noted in the nerve cells of the most superficial molecular layer of cerebellum and appeared as swelling of nucleus and cytoplasm. Then followed similar changes and pyknosis in the granular layer, and later the Purkinje cells showed these changes. The tissue became vacuolated, taking on a spongy or honeycomb appearance, and by 10 hr after irradiation, necrosis was so extensive that cellular elements were obliterated and no longer recognizable. After 10-kr irradiation, similar but less extensive necrosis of nerve cells occurred. Examination of cerebellum several months later showed widespread destruction of ganglion cells and replacement by microglial elements; proliferation of macroglia and a coarsening of epithelial flber processes in the molecular layer were also evident. After 120 days the damaged regions of cerebellum showed a cell-poor, fiberrich scar in which macroglia were prominent. Blood vessels were dilated and their walls thickened. (H.H.D.)
- Research Organization:
- Univ. of Bonn
- NSA Number:
- NSA-16-028768
- OSTI ID:
- 4780771
- Journal Information:
- Beitr. pathol. Anat. allgem. Pathol., Journal Name: Beitr. pathol. Anat. allgem. Pathol. Vol. Vol: 126
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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