Radiosensitization of a Mammalian Cell Line with 5-Bromodeoxyuridine

Journal Article · Mon Oct 01 04:00:00 EDT 1962 · Radiation Research

DOI:https://doi.org/10.2307/3571174· OSTI ID:4780415

Delihas, N.; Rich, M. A.; Eidinoff, M. L.

BS>Some radiobiological properties of a mammalian cell line after exposure to the halogenated nucleoside 5-bromodeoxyuridine (BUDR) are described. Incorporation of BUDR into the deoxyribonucleic acid of mammalian cells in culture to the extent of 1% was measured after 3 to 4 days of in cubation with 6 mu g of the analog per milliliter. An in creased x-ray sensitivity of about 2 and an increase in extrapolation number of survival curves were observed after exposure to the above-mentioned concentration of BUDR. In contrast to this, an enhanced sensitivity slightly greater than 1 and a decrease in extrapolation number were found after thermal neutron irradiation and identical treatment with BUDR. Theoretical calculations on x-ray energy absorbed by BUDR-exposed cells show that a negligible increase in energy is absorbed per cell with incorporation of 1% BUDR, as compared to unexposed cells. For analogexposed cells, survival was found to increase when a single dose of x rays was administered 4 to 12 hours after cell plating. For unexposed cells, survival was found to be constant during that same period.

• Research Organization: Sloan-Kettering Inst. for Cancer Research, Rye, N.Y.; Cornell Univ. Medical Coll., New York
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-032790
• OSTI ID: 4780415
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 4 Vol. 17; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ABSORPTION
ANIMAL CELLS
BIOCHEMISTRY
BROMINE COMPOUNDS
DIAGRAMS
DNA
IRRADIATION
MEASURED VALUES
NUCLEIC ACIDS
RADIOBIOLOGY
RADIOSENSITIVITY
THERMAL NEUTRONS
X RADIATION