THE EFFECTS OF 5-BROMODEOXYURIDINE ON RADIOSENSITIVITY, DNA CONTENT, AND FINE STRUCTURE OF HELA CELLS
Thesis/Dissertation
·
OSTI ID:4715304
HeLa parental cells were cultured from one to seven days in complete growth medium supplemented with the thymidine analogue 5-bromodeoxyuridine (BUDR). Some of the cultures were additlonally treated with 5-fluorodeoxyuridine, an inhibitor of thymidine synthesis. Following one to six days of growth in BUDR- containing medium, the cells were exposed to x radiation or ultraviolet light. Cell survival was evaluated by the colony-count method of Puck. Feulgen cytophotometric measurements of DNA content were determined in conjunction with mean cell generation times and growth rates of cell populations. Electron microscopic observations of cell structure were made following x irradiation and/ or cultivation in BUDRcontalning medium. HeLa cell sensitivity to both x- radiation and ultraviolet light was found to increase following cultivation in BUDR-containing medium. Increased radiosensitivity of HeLa cells was apparent following incubation in BUDR-containing growth medium for a period of time less than that required to complete one division cycle, i.e., about 24 hours. Exposure of HeLa cells to radiosensitizing concentrations of BUDR resulted in decreased cell viability, decreased rate of cell division, increased nuclear size and DNA content, and alteration of cell structure. These multiple manifestations of the biological action of BUDR provided evidence to support the conclusion that cultivation of HeLa cells in the presence of radiosensitizing concentrations of BUDR does not result in true chemical radiosensitization. (Dissertation Abstr.)
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-17-024872
- OSTI ID:
- 4715304
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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