X-irradiation and Diphosphopyridine Nucleotide (DPN) Levels in Ascites-tumour Cells and Liver Tissue of Mice

Journal Article · Mon Jan 01 04:00:00 EST 1962 · International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine

DOI:https://doi.org/10.1080/09553006214550691· OSTI ID:4775110

Schneider, M.; O'Bourke, F.; Redetzki, H. M.

Mice with Ehrlich's ascites tumor received total-body x irradiation with 900 to 5000 r. Concentrationa of oxidized DPN were analyzed enzymatically with yeast alcohol dehydrogenase in liver and tumor-cell homogenates 2 hr and 12 hr after irradiation. After 5000 r, tumor cells and liver exhibited but little decrease of DPN, 5% and 7% respectively at 2 hr, and 12% at 12 hr. When animals were irradiated before or after intramuscular administration of nicotinamide (170 mg/kg), significant alterations of steady-state concentrations of DPN were observed. After irradiation, average decreases of DPN in tumor cells and liver were 22% and 27% respectively (2 hr) and 16% and 17% (12 hr). It follows that x irradiation within the stated dose-range moderately inhibits DPN-synthesis or activates DFNbreakdowm. The effect is compensated at normal cellular oncentrations of DPN, but is demonstrated if DPNynthesis is activated by administration of nicotinamide.

• Research Organization: Univ. of Texas Medical School, Galveston
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-000068
• OSTI ID: 4775110
• Journal Information: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, Journal Name: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine Journal Issue: 2 Vol. 5; ISSN 0020-7616
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CEREALS
INSECTS
LIFETIME
PARASITES
RADIATION DOSES
RADIATION EFFECTS
SEX
X RADIATION