Dry, High Resolution Autoradiography

Journal Article · Sun Jul 01 00:00:00 EDT 1962 · Stain Technology (U.S.)

DOI:https://doi.org/10.3109/10520296209117744· OSTI ID:4774412

Branton, D; Jacobson, L

Sections of freeze-dried, paraffin-embedded tissues are placed on pieces of thin sheet Teflon backed by a felt pad. The sections are then pressure- mounted on dry photographic emulsion. After suitable exposure, the sections are firmly cemented to the emulsion with 0.45% cellulose acetate in a 10 : 1 mixture of 2-butanone and acetone. This prevents the specimens from falling off or moving during photographic processing, though the tissue can be stained through the cellulose acetate binder. The method was tested with tissues containing H/ sup 3/-labeled DNA, and it produced resolution comparable to that obtained with standard liquid emulsion or stripping film techniques. The technique was also successfully used to study the localization of Fe/sup 59/, C/sup 14/, Na/sup 22/, Rb/sup 86/, P/sup 32/ and Ca/sup 45/ in pland cells. The results indicated substantial reduction of the redistribution and leaching of soluble substances noted with the standard wet autoradiographic techniques. (H.H.D.)

• Research Organization: Univ. of California, Berkeley
• NSA Number: NSA-17-001199
• OSTI ID: 4774412
• Journal Information: Stain Technology (U.S.), Journal Name: Stain Technology (U.S.) Vol. Vol: 37; ISSN STTEA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACETATES
ACETONE
BIOLOGY AND MEDICINE
BUTANE
CALCIUM 45
CARBON 14
CELLULOSE
DNA
EMULSIONS
FILMS
FLUORINATED HYDROCARBONS
IRON 59
KETONES
LABELLED COMPOUNDS
LOW TEMPERATURE
NUCLEIC ACIDS
PARAFFIN
PHOSPHORUS 32
POLYMERS
QUANTITY RATIO
RADIOAUTOGRAPHY
RUBIDIUM 86
SHEETS
SODIUM 22
THICKNESS
TISSUES
TRITIUM