HIGH-RESOLUTION AUTORADIOGRAPHY WITHOUT LOSS OF WATER-SOLUBLE IONS
A technique devised to provide a faster and less complex method for mounting paraffin sections directly on autoradiographic plates is described. By combining the procedures of flattening, mounting, and de-paraffinizing the sections, a new technique was devised to study the distribution of highly water- soluble S/sup 35/ in barley roots. The resolution obtained and the reproducibility of autoradiographs of subsequent sections indicate that intimate contact between sections and emulsions are obtained with the technique. Another advantage is that sections stay in contact with the emulsion, which enables precise localization of the activity in the roots. The technique is simple and probably can be used for similar types of tissue that contain radioactive material that is insoluble in toluene. (P.C.H.)
- Research Organization:
- U.S. Soils Lab., Beltsville, Md.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-028686
- OSTI ID:
- 4796280
- Journal Information:
- Nature, Journal Name: Nature Vol. Vol: 195
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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